TCAG Facilities
Microarray Analysis - Gene Expression and Genotyping
TCAG Facilities
- Biobanking
- Cytogenomics and Genome Resources
- DNA Sequencing/Synthesis
- Genetic Analysis
- Informatics
- Microarray Analysis
- Statistical Analysis
The Microarray Analysis and Gene Expression Facility offers a comprehensive set of microarray based services to researchers in the academic, commercial and pharmaceutical communities.
Please see our acknowledgment policy and terms of use.
We provide
- Free initial consultation to discuss experimental design to address all steps crucial for a successful microarray experiment
- RNA/DNA quality analysis using Bioanalyzer or TapeStation
- RNA Gene Expression analysis and DNA SNP genotyping analysis in Affymetrix or Illumina microarray technology
- Methylation analysis in Illumina microarray technology
- Statistical consultation services for simple and advanced data analysis
1. Affymetrix platform
Overview
Affymetrix oligonucleotide GeneChip® microarrays are available for both RNA analysis of gene expression and DNA analysis for mapping, association or genomic copy-number studies. The facility is an accredited Affymetrix Service Provider. For detailed information about this technology and further product information, please see www.affymetrix.com.
Pricing
| Gene Expression GeneChip | ≤ 24 Samples | 25-48 Samples | ≥ 49 Samples |
|---|---|---|---|
| Single Cartridge Array Format | |||
| Human Clariom D | $300 | $290 | $280 |
| Mouse Clariom D | $300 | $290 | $280 |
| Human Gene 2.0 ST | $340 | $330 | $320 |
| Mouse Gene 2.0 ST | $340 | $330 | $320 |
| Rat Gene 2.0 ST | $340 | $330 | $320 |
| Human Clariom S | $220 | $210 | $200 |
| Human PrimeView Array | $230 | $220 | $210 |
| miRNA 4.0 | $250 | $240 | $230 |
| E.Coli Genome 2.0 | $350 | $340 | $330 |
- Additional charges may apply for samples with less than 50ng/ul total RNA, when additional amplification process is needed.
- Gene 1.0 ST arrays are available for model organisms: (see detailed list on www.affymetrix.com). For pricing and processing please contact the facility.
Sample preparation and submission
All RNA samples have to be delivered or shipped to the facility in dry ice.
- Affymetrix sample requirements document. (Acrobat 173 KB)
2. Illumina platform
Overview
TCAG offers genotyping and methylation analysis on any publicly available Illumina microarray including:
- Whole-genome genotyping and copy number analysis using Infinium Omni arrays
- Focused genotyping arrays, non-human chips, etc.
- Custom genotyping using Illumina's iSelect assay, and other genotyping microarrays not listed in the table below, such as GSA
For more information on Illumina's suite of products, visit www.illumina.com.
Pricing
| Whole-genome Genotyping | <= 96 samples | 97-480 samples | > 480 samples |
|---|---|---|---|
| Human Global Diversity Array-8 | $170 | $165 | $160 |
| Human GDA + PGx-8 | $200 | $195 | $190 |
| Global Screen Array-24 | $102.5 | $100 | $97.5 |
| DNA Methylation profiling | |||
| Human MethylationEPIC array | $475 | $465 | $455 |
| Mouse Methylation array | $375 | $365 | $355 |
| Bisulphite Conversion | $35 | $35 | $30 |
3. RNA/DNA Analysis using Bioanalyzer or TapeStation
Overview
To verify quality and quantity of RNA or DNA samples for any downstream application, analysis using Agilent Bioanalyzer or TapeStation (Acrobat 56 KB) is available.
Pricing
The fee is $15.00 per sample for either RNA, or DNA samples. This fee is waived for any RNA sample being used in subsequent microarray experiment in the facility
Sample preparation
- Please provide an additional aliquot of approximately 5 µl RNA (approximately 200 ng/µl) diluted in water and shipped to us on dry ice with the test sample*.
(*if also submitting for one of our microarray services).
Samble submission
4. Sample submission for Microarray or Bioanalyzer experiments
Request your service using the Clarity LabLink server. For instructions on creating an account, placing an order etc., download the following document.
- Clarity User Instructions (Acrobat 95 KB)
The following forms are used to upload requests to your Microarray LabLink account. All forms are in Excel format unless otherwise indicated.
- Microarray Analysis (tubes and strips) (62 KB)
- Microarray Analysis (96-well plates) (86 KB)
- Bioanalyzer for Microarray (67 KB)
Samples should be delivered to:
TCAG Microarray Facility
Attention: Dr. Chao Lu
The Centre for Applied Genomics
The Hospital for Sick Children
Peter Gilgan Centre for Research and Learning
686 Bay Street, Room 13.9410M
Toronto, Ontario
M5G 0A4, Canada
Facility Contact
Chao Lu, PhD
The Centre for Applied Genomics
The Hospital for Sick Children
Peter Gilgan Centre for Research and Learning
686 Bay Street, Room 13.9410M
Toronto, Ontario
M5G 0A4, Canada
Tel.: 416-813-8059, Internal: 308059
Fax: 416-813-8319, Internal: 208319
Email: